CR60372

CRIMIC CR60372

Gene CG43366

CG43366

Information

Donor Location Cytology FBti Stock number
pM37_p2 2R :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GGCTGCCAAGTATTGACACAGA GTGTTGCAATGGGATTAGGACG

Images

Construct Design Sequences

sgRNA sequence ATTTTCAGTGATACTCGTAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCATTCACAGCCGCTCTAAGATATTGCGAATCTCTAATTTCCGTCAGGTATATGCATTCCGCAATGCACCCGTCCATTTTTGGTAGGCAGTGCCTTCGTACTCCCTCGCCTAAGACCAAATTGCCAGGCACTTAGGGCACCGCACCGAAAACATGCTTTACTGCAAAGACGCCGACTTCAATGGCATTACTTCACCCACTCGCCTCATGTGGCTGCTCGTTATTATTAGCATAATTACAGGCGCATTAGCGTGCCGGGTTTCAGGTGCTTGGCAATTTGCCTGCTGCCAGGACGCCTGATTATGACAGGGCCTAAAAGCGAGACGCATCCACAATGCACCTCCTGCAGTGCCGCTTAAGCGCTTAAAGTTGCGCAGTTTCTTTAAGGGTACCGCGTTTTATGCAAATTTCCCACGGCATTCTTTACGCTCGCCTTCATTAACATTTCATTAATTAGTTTTTTATTCCACTTTGCAGCTTTTTCTTGATGTTTATGCATCGTCCTTCGACACCAAGCACCAATCGGATTCAGTGAGCCAAAGGTCCTGACCTCTCGAAAGCAGAAGGAAGTGGAAAGCCATTTTAAGATTAGAACGAGGCTGAAACACAAACAAACGCCCGGGACTTCACCACTCCACAATGCGTCTACCGCGCACTTTTCCAGCGCTTTGGCTCTGGGCCGTGCTACCCTTGGCCACCCCCGGCATTCTGGGCTATCCAAACACCCAGTCACAGCCGGCACTGGTCACCTATGTGACGACCTCGTCGTCGTCCAGTCAGCGGGGCCACTCCGCCAGTCCGCACCTACAGCCCGCATTCGTCTATGAGTCACCCCTTCCGGAAGAGGAAGAAGGTTCCAAGCATTCACTGGCCACATCCACATCCTCATCCACATCCGATGTCCGAGACGAGAAGACGACCGAGTCGTCAAAGAAAGTCATAGGTTCCAGGTGAGTACACACACGAGTTTTATACTGGGACTTACGCTCATCAGAATTAAGGAATCAACACTGAACTTCTTACCAC CGAGTATCACTGAAAATAACTTCAAATATCCGACTCGCCTAGTATAAATAATAAATATAGTATCGGAATTAGATTAGTTTTCCGAAAAATAAGTTAAAGAAACAAACTTAAATATTATCAGATTGAGCTAGATTATATTGTTTTTAATCTCCAATCTTATTATTTTCTGTGTACAAGGGTGCGCTATTTTTCCACTTCTAATTTTTTTGCCCTTTGTTTGTTGTTTCTATCGGTGTTGCTGTTGCTAAGGAAAATCTTTTGTTTGCACAGCTTTTTGCAATTTTTGCCACTAATTGAAATAGAATTTCTGCTTTGTTGTAGCTGCCGTTCCGATTGAAAAGCGTGGGTTGGTCAGGCTGGGTCCTCGCCTTAGTATTTTTAATTAATTTGCTCTCAGGCCGCAAAGCGTGCTGGAGCGGGCAGAAAAGCATAGCATACTTTGTGGCACGCGTTTGGAATCCCATTGAGTTGGCGTTGGCTCTAAGGTAGTAGGTGGTAGCTAGGCAAAGGTGGGCTGCAATCGAGGGCCAGACAACACTGTCCAAATGAAGTGCTCATTAACTTTAAATCAATGCTAATCAATTGGGCTTATGTGGGCGCGATGTTATTGAAGTGGGCCCCAAAACGGCTTAACATTAAGCAGAGAGAGATAGAGATGGAATACAGCAAAACAGAATTTATTGGCACACTTTTCCTTTTCTGTGGCAGGCCCGTCGTTTTCTCAACGGGCCTTAGATCGTTCATATTAACGGGTTACGGTTTTTTGATTAATTCCACTGTTCGCAACCAGCGCCCTTCTGCGTCAGCTGTTCGCCCTCCTTTTTTCCTCCTTTTACCGTTGGTAATGACTGCGAGCTGTTGCCAGTGATGGTGATAACGATGATAATGAAATTGATGACTCGCAGCTCCGTGTTGAAAACTAGCAAAACAAGTCAAGCGCAACTGCAATACTTGTATTTACATGTATTTTTGATTGTGCTGCAATGCCCTCTGGATTCCAATTAAAACCAATCCGTGCGCTTGACATTCTTTGGGTCTTGTGCCACAAAACG
 
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