CR60380

CRIMIC CR60380

Gene l(3)72Dp

CG32155

Information

Donor Location Cytology FBti Stock number
pM37_p23L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TGTTCTATAAATACACGTGTCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GATCGCACACACACACGTCCGCCTGTTGCTATCTATCAGCTGTTAAGTAAACAATAACACAGACTTCGTTTGTTTTATAATGTGAATAAAAATCGCGACTGAACAGTTATGATCGATGCAATTAGTTAATTATCCAATAGTGCCCGAATAAAACAGGTTTTTATCCAACTTTCGATGGAAGCCCCAGGAGTAATTCCCCTTCTCATTGAGCACCCTGTGCCACGGTCACAATACTTTGGAAAAAAAATATAATCAATTAATCGCAGTACTATCTGCTGGACTCAAGTCGCCAGGGGTACACCCCCCGAAGGCCCTAGAAATCACACATCGCAAACTCATGCCCACGCACACAGCCTGAGCCATTTGTATAGGTGAGGAAAATCGTCGCACCTTATATAAATTCAAATCGACTGTTGCCTCAGCGGACAGTTCCTATTAATTGGTCACCGAGGTTGGACTCACCTGTGCCCTTGCCAAGACTTCCCGTTGGGAAATACTTCTGGCAGCCGGAAAACTTGCGAATAATTAAAACAACAAGCTAAGAAAGAATTCCCTGAGAAAGCTAGCTTACCGACTTAGCGTGTTTTTAAAGAATTACCTGTGGAATAATCAATAGCTCATAACAAGGTACTTAGGAAACTGAAGATACCTCCAACCGAACCAAAGAAAAACGGATATTTCGGAAGATAAAATACTCGTTTTAAGATCTAACAAAATGCAGAAGTTCTCGATCAGCTTGCTGATCTTCATCGGAGCTCTGTTGGCCGCTTCAGAGGCTGGCATCTCATCGCCCATCATAGGGGTTCTCACACAGGAAGTTTACGTCGATGGTTTGATCTCGAGACATTTTGATAACAAGACCAGCTATATAGCCGCCTCGTATGTGAAGTATCTCGAAGGAGCTGGAGCCCGAGTAGTGCCGATTTGGTAAGTGAGCAGAAAGATTCAAAGATCTTATCGTGACCGGTAATTCTGCCACTGCTCGATAAGGTCGAATAGGGTAGGGTGGCATTAAGTATTCCGAC GATTTGGAGACCGAAATTTCGTTGTATTGATAAGATTGCCTGAAATCAATTAATAAACAATTTTCATACAAATACCTTTGACAGGATCGGTCGCAATCGCAGCTACTACGACGATCTCATGCGCAAGATAAATGGGTAAGTATAAATATACTTTCTAGGAAGTTCCCGCATCTAAATTTATTGCTACTTCAATAGAGTCTTGTTGCCCGGTGGAGCCACTTGGTTTAATCAGAGTAATGGGTATGCGGATGCGGGCGAACACCTCATCCACTTGGCCATTGAACTAAATGATCAGGGCGTCTTTATGCCGGTGTGGGGCACTTGTCTGGGCATGGAACTATTGGTTTACAAGCTGGCCAACGAAACGGAACACCGCATTAATTGCGAAGCGACCGGCATGGCCGTGCCTATGGAGTTTAAAGAAGGTATAGTTTGTTATATGAATTTATATATTCCGAATGCTGAATCTACATATTTTTATCTAGACTATAAGAAGAGCCGCCTGTTTGCATCCATCACCGATGATGTGGTGGACACCATGGTCAAAGAGAATGTGACCTATCACTGGCATCAGTTCTGCTATACGGAAAAAGACTTCGAGCGGGATCTACTAAACGAAACTTGGCGGGTTATGTCCCTAAATCATGACTGGAATGGCGTTGAGTTTATTTCCACCGTAGAGCACATAAAGTACCCCTTCTATGGAGTACAATTCCATCCGGAGAAACCGCTGTACGAGTTTACAAAGACAAGCATTCCCCATACCGCAGCTGCTGTGTTAAGTGGTCAATTCTTCGCCGATTTCTTTGTGAGTGAGGCGAGGGAGAGTAATCAGAGCTTTTCGAATGCCACGGAGCAGGCCAGGACGCTCATCTACAATTATAAGCCGGAGTATACTTCGATTCTGGGCTCCTCGTACATACAGCAATACCTATTTACCAATGTTGAAATGGAAATACCCGAAATTCCGGAGATCCCTGATGAGGGCGGCAATAACGATGGGGGTAGTAGTGGCGGCTACATTCCCATTGTTGTACCAGGCGGTGACGGTTCTACCACTATGCCTTTACTAAG
 
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