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CRIMIC CR60397

Gene pasha

CG1800

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p1	3R:31617977..31622217	100D1-100D	FBti0226900	97201

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATACCTGGAACCATCACTAACCCC	TGAACTCATAGGTGGGCTGCGTTT

Images

Construct Design Sequences

sgRNA sequence	GAAGATGGCCATCAACTTCGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

GTTTCCGCAAAACTGCATGTGGTGTTTGTTTCGCTTGTTTGGTTATTTTTCCCAACTAACATCCTCTCTCTAAAATCTCGATTTCGTACTTGAATTGCGCAGCTTTTGCTTTGGGATTTTGTCCGACAATTAGGCAGAATCCATAGCGAAAGACGCAGCAAGTAGCATCGTTTACAATCCGCGAAAGTGCAGTCGCAGAATTTTCCGGCGGGGCTTCTGGCTTAACCAATGTGGCACTTCCAATGAGAAATCAATTGCAAGGCGTTGGATGCTCTCAGTATTGGTTATTGGCGAGATTGGCCCCGGGACAGCATTACTAGACAGTTCCGATCAGTCCGCACTAAACCCCTCCCGCTTTAGACACACACATCCAATACAATGGCGGAGAAGCCGCTGGCCGACTGCCCGGACTCTGGGCAGGTCCGCAAGCGTCCCCGGCTGGAGGACGAGGAGCAGGAGCTCAAGGTGGCCGGCTTTTCGCTGAGCGCCATCGAGGAAATGCGGCGGACCAGGGACAACGAGCTGCAGAACGAACCCATGGACCAGCAACTGCGACAGTTCCAGGTTCTCGACGAAGTCGGCTCTGGCAGCGATGAGGATGATGAGTCAGGTGAGTCGGAAACGAAGCTCGGATATTCACTCAGGTCATCCAGATCACAGGCTGTGCAATTACCACCTTTTGTTTCACAGTGCAGGCGCAGTTCTTATCTCAAGATCTTGGGGGCCTATCATTGTGCAAATACAGGAGGCTGATAAAAGAGTCAGCCGGCAGGTTAAACCCTGATATAGCAGCTTCAGTTTTCCTTTATCTTATTGTAAGGCAGGTGGTGGCAGTTTTGTGCGGACAGTTCCATGCGCATGATACCCATTATCCGACTGGCTTGTCCAGTTGCGTCAGGTAGAGCCAGTAATAATGAAGGATCACTTTAATTAAACATCAAAGAGCTAGACAATTAGGTGCCAATGGCATTCATTCAAATATTCCGTGAAGATGGCCATCAAC

CGCGGCTGGTTTTCCATTTCCCAGACAAACAGACAACTTATTTATCCGTGATTGTATCTCCTTACATGGCAACCAGACAACGCTAGCGAGCAGCAGGGCGGAAGGTATGGAGAAGGAGGCGACGAGGAGGAAGAGGCGGCTGATGGTGACGGCGAAGAATACGATTCCTCGGAATTCGATGATGACGAGATCGAGAATCTGCTGGACGAGAAGCTGCCCGAAGAGCTCCGCGAGTCAAAGCAGCCCAAATACGAGCAGCGTTTCAAGACTGTGCTGGAAGGTCAGTGCTTTTCGTCGAACCAAGTGTCCAAGATGACGAGTATTTTAATGTGACTACTCTTTTAGAAAAGCGCCTCAACCACTTTGAAGTCCTACCCGAGGGATGGGTGCAGGTGACCCACAACAGTGGAATGCCGCTCTTCTTGCATCGCAAAACTCGCGTCTGTTGCGCATCGAGGCCCTATTTTCTCGGCACTGGAAGTGCCCGCAAGCATGCAGTGCCCTTGGGAGCAATCCCCTGTCTTAACTACAGGCGGGCTTTGGAGGAAGAGTCGGAGGTGGAGCAACAAAAGACGGATGGAGCGCCGCCAGTGGCTGTATGTCCCATGGCCGGAGCTTCCGCTTTACCGGCCGAAGCCATGGACGTGGAGGGAAAAGAGAACAACGAAGGCAGCACCGATTCCAAAACCCAACCAGATGGAATAACTGCCCTTATGCCGGCTGCTAAAATTGTTACGGTCAACGAGAATACCCAAAAGGAATCCATTACACCGGAGCAACTAAACACCTACTGCCAGAAACTCTTCAAATTCAAGGTGATTCGTGTGCTGCGATTTCGGAGTTGGAATGCTCGCCGCAAGTTCACCAAAAACCGCAAACACATCAAGAATATTCAACGACCCACGCTGCCGGATGGCACTAAGCTTATTAAGTTCCCAATACTAGCGAATGCTGGCGAAGGATCAACGAACCCGCGCGGTCGGAAGGAGTGGATCATGAATCCCAATGGCAAGAGCTTCGTCTG

 

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