CR60423
CRIMIC CR60423
CG2774
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37_p0
| 2L
:.. | | | 94441
|
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TCTCCTGTCTTCTCCTAAGTGGCT |
CATTTGTGCGTACTGCCAGTTCTG |
Images
Construct Design Sequences
| sgRNA sequence |
AACGGGAGCTCAATTACTGGGGG
|
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
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|
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| Amplicon 5' |
Amplicon 3' |
| GGAGACCAAGAGCTGGAAAAACAAGCAGCGGCAACAGAAAGTTGCAATTGCAAAAATTGAAAATTTTCCGCATAAAAATATGCCCCGACAAGGAAGCAAAGGAAAAATCGCAGAAAATAGCAAGAAATCTTACTCCCAGCCAAGATGTCGACAAAGTATCGTGCATGTTGTTGGCAAAATGTTTATTGTCGAAAAGTTAAGCAGAATGCAGATTGCCACAGAAGAAATGTAAGGTCGCAGAAGGTGCAGAACCTCTTAGTTTATATAGTTATTAGAGCTCTAATAATCTAGCAATGTTGTGTCAACACTTGCCTTTGAATTATAAATTTACTTTACTTAAATTTTTTCTTTACGCAAACAAAACACTTTTACTTGAACATAAGTCTTCTTTATTTATTAGTCTAATGGAATTTCCATTTCGTTGCTGAAAAGAGTTTCTTGTTTGTGCCAAAAAGTTTCTGGTTTGAAAGTAATTAAATCCGCATTTACTTAATCAAAAACAATGCACAAATTATGAGCAACCTTTTTTAATTTATGGAAGACAATGCTTCCAAGAATGTTTACAATATCAGAGGTCGAATATTTATGGAAAGTAATTAAATATAATACTATTTGCATGTACGTTTTTAAGGTCCAGGATGGTAACGGTATTTTTCAAATTTAAGCGTGATTACCGTTCCACATGCAGTATTTTCAACGTTCTCTTAAATACTGAAAGCTTTCGGTCAGCTTTCCACCATCTAATTGTTTCAGTGGTCTGGTCACACTGCTGGGCGACCGAAACGAAACGAGTAAAAATTCCGATAATTACAATTAATATAAAATAAAAACGCGGAACATAACGAACAAAAATGGAGGTGGAAAGCCCGGAACACACGCGCGACTTCGCCGAGGTAGACATCAATAACGGTGCGGCCACAGGATCGGAAGACGAGGAGGAGGAGGTGCCAGCGCCCGGCAGCGTAACTCTGGATCGCAACGAAAGTGATCTCTTCGTGTCGGCCTTGAGTCCCAGCAGCATCGGGGATATACACGTAAGTTGGGGCACGAAACACGGGAGCAGCCCGCATCGACAG
|
GTAATTGAGCTCCCGTTTTGGGGCGTCGTCGGTCGTTTTATCAGCGCTCGCTTTGTTTGGTTTTGTGGCTACGTCACGAGTGCCGCCCACCAGCAAAGCTTGTTGCTCCATAGAAACACAATGAATTTGCGTCTTAATGCATTCCCCAATCCTCTGCAGCCGCTGCAGGAAGTGCTCACCGACGACGGCGACTACTTCATCAGCATCGTGGTCTCGGATCCGCAGAAAATCGGCGATGGCATGGGCTCCTATTTGGCCTACAAGTGAGTCACCCAGTTCCTTATCATAGAAAACATAATGCATTACAACAAAATTCTTATCGCCTCCGCCAGGGTAACGACCAAAACGAACATTCCCAAATTCAAGCGTAGCGAGTTCAGTACTCTGCGCCGCTTCAGCGATTTCCTTGGCATCCATGATCTGCTTGTGGGCAAGTACATGCGGCTGGGCCGCATCATACCGCCGGCGCCCTCCAAGAACATCATAGGCAGCACCAAGGTGAAGATTAGCCCGCAGCAAAGCGAACCGGGCACGCCCATGACCCAGGAATGGGTGGAGATCCGCCGGGCGGCCCTCGAGCGATTCGTCCACCGTACCGCCCAGCATCCGGTGCTGCGCGTCGATCTGGATTTCATGAACTTCCTGGAGAGCGACCAGGAGCTGCCGCGTTCAGTGAATACCTCTGCGCTCAGTGGAGCCGGCGTCATCCGCCTGTTTAACAAAGTTGGCGAAACTGTGAACAAGATTACCTACAAAATGGACGAGAACGATCCGTGGTTCGACGACAAGATCACCGAGGTGGAGAGCCTGGACGCCAATCTGCAGAAGCTGCACAATGCCATGAAATCGCTGGTCACGTCGCGTCGTGAGCTGTCGCTGCTCACCGGCTTGGTGGCCAAGTCGGCGGCCATGCTGAGCACTTGCGAGGAGCACACAGGCCTCTCCAGAGCGCTGTCCAACCTGGCGGACGTGGAGGAGAAGATAGAGCTACTG
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