| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pM37_p1 | 2R:24092079..24098636 | 60B9-60B10 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK |
 |
| sgRNA sequence | CTTAGATATTTAAAGTGCTGTGG |
| sgRNA2 sequence |
| Amplicon PCR 5' Left | Amplicon PCR 5' Right | Amplicon PCR 3' Left | Amplicon PCR 3' Right |
|---|---|---|---|
| Amplicon 5' | Amplicon 3' |
|---|---|
| GTGTGACCGCTCTTAAAAAAGCCAAAATACCGATGAAAATTGTGGTATTTATAGGCTTATTTTAAAAGTCTATAGGGCCACTCTGTTTTCCCAACGAAACGGAACACTACAATTCGACAACAGTTCGGCTTTTCTTTTTGCACTATTTCGGATAAATATGGTTTAGGCCAGACGGAGCAATAACTAAGATGGAATACGAATCTTCCGAAATCAGTGGTAATACGCGCGGTTCGCTGACACCAAAATCGGCAGTTGGAGGCCGTGCTTGTTTCCCAATTGAGCATTTGTTGTTTCGCATTCTGCCAACCATACATACATACACGCATATAAACAATTATTTATTTATATCTGTGCGGAAGAACCAGCCATATAGGCGAGATTCGGGTGTATTTGGCATGCTGAGCGTAGGGTACGGCTCCATACCTAATATATCCTCCTGTACGGGGGGTCGGTCCAACACACATGCATGTCCCGCATACATGTTTGAAAAATCGAAATGGCCCGAATATGAATCGTAAATAGTGGTGGCCAATAGGAGAATGCGGAAAATATGGGAAAGCTGTGCATGCTTGGAAATATAGCGGAATCCATTAGGAGTACGGCCTTCTGCTTGCGCCCATTGTTCTCCGGTCTACCTTCCCCCACGTTTCATCTTGGTTTTCCATAGCCATAGCCACAGCCATAACCCACCCGTTTGAGCTGATTTCTGCCCCCCGAGCCGCATTTCTGAATTGGAAGGAGCCAGGTGTCTGCGACGTCAGTGGGTGCGGCATTGTTGCGGGCTGGCAAATGTTTGCGATGCATGTGTTGGTCGGCTTTTTGGGGTAATACAACAATTGCTCGCGCTCTTGGCCAAAGGAGGACAGTCTCCGAGTTTTTATCAGCTTACATACATATGTGTTTTTTAATGATGCCAAGGATACTTACGTATTAGGTTTCCACAG | CGTAACTATTCGAGCATCAAAAGTGGTTATATCTTTTAGTGACTCACGCACCAAATTCTACGTTGCAGATATAACCACAGGAAGCTGCAAGAAAAGGCTCACATTTCTGAAATGTATTCTAAGCGACACCACAAGCGATCAAAAATGGGCGGCCGAGTTCGGCGAGGACACGGAAGGACATCAGTTTTCGTTGGACTATCTGCTGGACACCTTCATCGTTCTCTATGACGAGTGCAGCAACTCCTCCCTGCGCAGGGAGAAGGGCGTCTCTGATTTCCTCAAACTATGTAACTATGCTTAGTACAAACCAGATGTGCTATCGAGTACTTAAAACCTGAATTACCCCACAGCCAAACCCTTCGTGCACATAGTGCGAAAGCTTCGACTAAGCCGAGATGACTTTGACATACTCAAGATCATCGGACGCGGTGCCTTTGGAGAAGTGTGCGTGGTTCAGATGATATCCACCGAAAAGGTGTATGCCATGAAGATTCTGAACAAGTGGGAGATGCTGAAGAGGGCCGAGACGGCCTGCTTCCGCGAGGAGCGGGATGTGTTGGTTTTCGGCGACCGGCAGTGGATCACCAACCTTCACTATGCCTTTCAAGACAATATTAACCTGGTATGTCAGCACTCCTATTCTCCGTTCTTACCTTATTAACACCTTCCTTTCAGTATCTGGTGATGGACTATTACTGCGGCGGAGACCTGCTTACATTGCTCAGCAAATTCGAGGACAAGCTGCCCGAGGACATGGCCAAGTTCTACATCACAGAGATGATCCTGGCAATCAATAGTATTCACCAGATTAGGTACGTTCACAGGGACATCAAGCCGGATAATGTACTGCTCGATAAGCGCGGTCACGTGCGCCTGGCTGACTTTGGATCCTGCCTGCGCCTGGACAAGGATGGCACCGTACAGTCCAACGTGGCCGTGGGCACACCCGACTACATTTCCCCGGAAATTTTGAGGGCCATGGAGGACGGAAAGGGACGCTACGGCAC |