CR60442
CRIMIC CR60442
CG1851
Information
Donor |
Location |
Cytology |
FBti |
Stock number |
pM37_p2
| 2R:.. | | | 94454
|
PCR Verification
PCR check relative to sgRNA sequence
HA-L |
HA-R |
Forward |
Reverse |
multi band (weak) |
OK |
GGGATAACTCGATGGCATTTGTGG |
CATTGCTTGGGTGAAATGGTGGAC |
Images
Construct Design Sequences
sgRNA sequence |
TGGAAAATGGCAAAGTTACACGG
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sgRNA2 sequence |
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Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
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Amplicon 5' |
Amplicon 3' |
CAGCGCAGTGCCAAAAGCTCTGTGTTGTTGAAAAACTCGACAAAGTCAAAAAAGTCGAAAAAGTCAAGCTGCTCTTTGTAGGCCGCTCGATACAAAGGTAAAAAGAAGTTGGCCCAAACAAATTGCCCGAAATGGGAAAAAACAAATCGAGCACGCTGGCGAGAGCGAGATAAAAACACCGGGACAAACCGGCCCGAGCGTTCAGTTCTGTGCCGGACGACAAAAGCGGCGGATCACTGTTGGATTTCATTTCATCCGGAGCACTTGCGAATAGAACGCGAAAGTCCCGAGCAAGCGTTAGTTTTTGCATACAATGGAGTACCTGAGCTACGTGTACGAAAAAATCTTCGGGGGCAATCCTCCCACGGATGACACGCAAGGGGCTTTCCACACGTGAGTATCAGCAGTAGCAGCTGAGGCGGAAAAACCCGGCAGAAAACACCTCTGACCATTGGGCTTTCCCACTTTCAGGCCCCGCAAGGTCATCTGCTTCGGCAACGTCCTGCTCGACCGACTAGTCCAGCTCGAGGATCCCCAACTCCTGGAGCGGTTCGGCCTGGAACTGGGCTCCAAGGGTGAACTGGACATGGAGAAACTCAACCAGCTGGCAGCGGAGGCTTCTGAGAGGTGAGTACCTTTGTATCTTGCCCGATTGTTTCATTATCTTCAATGACTGATTGTAAGAAATTGTACAATTGGACCCAAGTTATGGTTTTTAGTTAGACTGATTTTACATATAAGTAGTATAGTTAAGGTCTTGCAAGTATAATTAGTATCACCTTTCAAAGAAATTGTGAAAAGATACAAAGAATGAACAACAAATAGGACTGGGGCTACAAAGGCTGTTTATACATAGCACTTTTGTACACAACAATCAATCTTTACTTAAGAAGTTAAGAAGGGCCTTCTTTCTAATCTCTAATGACAGCAAAACTGGTTTTTATACCTGTACGAAATCGTCTTACGAAGTAGGAAATGG
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CACGGTTATTCTAAAATTACAAGGGTTTAGTTAGTAACTACATACATACATAATAAACAACCTTTGCAAAGCTATACTTACTAATCCGAGTTCAATCCAATTCAGCTCTCGGTGCCTTACAAACCCGGGTGGCTCTGCCCTCAACACTGCCCGCATCCTGAAGCAGCTGGGCACAGACGCGCTCTTCTTTGGCGCCGTGGGAGCCGACAAGCACGCCGAGGAGCTGCGCCAGATCATTCGGGATCGCGGCATCGAGGCCAGGCTGCAGACCGTCGAGGATGCGCACACAGGGCAGTGCGTGTGCCTCATGTATCAGGACAACCCCACGCTGTACGCCAACATCGGCGCCTCGGCTCAGTTCGAGGTGCAGACCCTGAGCCACGCCGTCAGCCACGAAGGCCAGAGCTTCCTGCGACCGGTGGAGCGCAAGCAAATCCTGTACGTGGAGGGCTTCTTTGTGCCCCAGAGGAGTGATGTGTGCGATTACATAGTGCAGCACCTGGTGCGGGAGCGGCGACGCCTGGCTCTCAATCTCAGTGCACCCTACATTGTGAGGAAAAATCACCAGGCTATGATGAAGCTGGCCCGGGCCGCCTTCTTCCTGTTCGGAAACCGACAGGAGTTCGAGGCGCTGGCCGAGGCTGCGGGTGGGTTCCGAAACGTCGACGAACTGGCGGATCATCTCCTGCAATCCGGTGGCACCAAGGTCATCTTCGTCACCAACGGCAGTGCCGGCGTCCAGGTGATCACCAACTACGTGGAGGAGCTGGCTCCTCCCGGACCCGTAAGCTTTGAGGACTTCAGGGCGCAGCGCGTAGAGCAGCTGGTGGATGCCACTGGGGCCGGAGATGCTTTTGTGGCCGGATTCTTGCACGCCTGGCTGGAGAAGCGCTCGCTGGGCGAGTGCATCCGAATGGCCTCCAGCGTAGCCGCCAAGGTCGTCACCCAGGTGGGCTGCAATCTGCCCTAGACCGACGCCGTGCATCGCCACCTCAGATAGCG
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