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CRIMIC CR70008

Gene CG8027

CG8027

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:9204410..9207124	45B3-45B4	FBti0220375

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTTCGGTGTCCAATTAGCGCAGTC	GACAGCAGCCAGCCAGTGAACATAAT

Images

Construct Design Sequences

sgRNA sequence	GCACGGCCACACTGTCCCGCAGG
sgRNA2 sequence	CATATCACAACATTGCAGAAGGG

 

left_200bp	right_200bp
AACCGGTTCCGTTAATTCCACATGTTGATTCTTTAAAACCCGTATGTTCTATTTCGCACTGCTTACTAATAGTTTAATTAAATATCTAAAGAAATTCGGGTTTTATACTGATTGCTTTTATAAGCTCGATTTGAACCGAGTTTTTATTTCAGTCTTTCCAGTTAGTAATTTTACGCAGTTCTAGCACGGCCACACTGTCC	TGCAATGTTGTGATATGTTGTGAACATTTATGTAAATTGGCCATTTCTGCAAGCGATCATAGTCAAATCTAGTTGTAGGCCATGTGTAAGGCTATTTTTCTAGTTCTTTAGGTGCATGCTAGAGGCTACGTTTAGAAATAGTCATTAAGAATGGATGCGTAGTGAAATTTTTTAGAATATTTTGTGGTCCAGCTTTTGCA

 

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