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CRIMIC CR70009

Gene Lip3

CG8823

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:13370240..13371904	87E8-87E8	FBti0216201	92686

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGAGACCCGAGTACAATGACAAGA	CGTAGGATTGCATCTGCTTCAAGACC

Images

Construct Design Sequences

sgRNA sequence	GAGCAGCATTATAAGTTACATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTGGAGGTCTGCGGATCCATGGAGTTCATGCCCAGCAACAAGTTCAAGCTGAATTTGGGCATAGAGATGTGCCAGGCCACTTCTCCTTATGCCGACATGTGTGCCAACGAGATCTTCCTGATTGGAGGCTACGATACCGAACAGCTGGACTACGTAAGTATCTGGAAACGAACTTGGTCCTATAAATTATATTTCCATGT	AACTTATAATGCTGCTCTCCATTTGGTCAGGAACTCCTTGAGCACATCAAGGCCACGTCTCCGGCTGGCGCATCGGTGAACCAGAACTTGCACTTTTGTCAGGAATATAACTCTGGAAAGTTCCGTAAATTCGACTACACCGCTCTCCGTAATCCCTATGAGTACGGCAGCTACTTCCCTCCGGATTACAAGCTAAAGAA

 

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