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CRIMIC CR70020

Gene betaGlu

CG2135

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3R:31769293..31771848	98F1-100F5	FBti0220379

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCGATTTTTTCTACCAAACCATC	CTCATTTAGCCTCCTTAATTATGCCAC

Images

Construct Design Sequences

sgRNA sequence	TCTATCGAGTTACAATTTAATGG
sgRNA2 sequence	AACATACAAATACGGTCTCTTGG

 

left_200bp	right_200bp
CGGTAAACAATTTTTTTTATCATGAGTAGAAGGCCAAACCTAAACCTAAATTCTTTTAAGTTTACAATTTTAAGTTTATGTAAAAAAATTTCATATTAAATAATAGATGGATTTTGTTCACTATATTTGCGCTGGTCACACTGCTCTTATCAAAGGCCGGGTGAAGAAGAAGATGGTTCACAAATGTTTGATAACCATTA	GACCGTATTTGTATGTTATAAAGGTGCGAGTTATTCACTACGCATTACATTAAGCTTTATTCAAATTTTTCAGCAATGGTCTGTGGAATAATTTTTTTTTTTAAATATGTATTATCTTTTCTTATTAAATCATGATTCCTAATAGATATATTATCTTTTAAACGCTTAATCTTAGCTAATGATGGAGAAAAACAGATTTC

 

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