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CRIMIC CR70032

Gene Ppt1

CG12108

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:8688627..8690227	8A2	FBti0216204	92689

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTTGCAAGCAACTACTGCAGGA	ATTGAAACCACTGGGACTCCTTCG

Images

Construct Design Sequences

sgRNA sequence	GAATTAACACGGAAACGACAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTCGGTGGGCAGCACCAGGGCATCTTCGGACTGCCCATGTGCCCCACCTTAACCGAAAAGCCCTGTGATTATATCACGAGGCTACTGGACAACGCCGCCTACGCACCGTGAGTCTATCTGTTGTTAAGGAATCCTAATATAATATAATTCCTGGAATTTACATGCTTATTAATCCAACACCACCATGTCGCCATCCTTGT	CGTTTCCGTGTTAATTCTCTCTTCTCTCTTGCATACCATACACCCAGTGAAGTGCAGAAGGCATTGGTTCAGGCCACCTACTGGCACGACCCGATCATGGAGAACAAATACCGCCTGGGCAGCACATTTCTCGCGGACATTAACAACGAGCTCTTCATCAACAAGTTCTACATTGAGAATCTGCAAAAGCTTAAGAAGTA

 

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