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CRIMIC CR70036

Gene anne

CG32000

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	4:132220..143123		FBti0216207	92692

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGTTGCCTTTTCGATTAGGCG	CTCTGTTACCAAAACCTGTTCTGCC

Images

Construct Design Sequences

sgRNA sequence	ACAATAATGTAATAGAACGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCAACTATAGGAGTGTAATTGATTTAACTTTACTGTTCTTATAATTATACAAAATAAAACAGACAAAAATAAGCCAAGGTAGGCGTACTTAAATACATACATAGATAGAAGAAAATAGCAAATTATGACACCCCACAATGATTCAAAAGGTATGCACTCTTACATAGTAAGCGAATTTAAATTATATAACACGCCATCG	TTCTATTACATTATTGTTATTAGTATTTAAAGCTTGATTACTTATTTGTAGATTCAAAACCATGTATTGGACTATTAAATCCAGATCAGGAGGATCAAATGAAAGTATGCGGTTATCGACGTTCTCTTATGCGAACTGGTTTTTGTTGGGCATGCATCTTCTTAACTGGAGGTCTGTTGCGCCTCGTGTTACATTGGTGG

 

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