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CRIMIC CR70050

Gene vkor

CG33544

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:16778365..16778994	53D8	FBti0220386

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GATGATGTTGTCGCAGTCGCTAGT	ATACCACCAAGCTGCTCTGGATCT

Images

Construct Design Sequences

sgRNA sequence	TTACCCAAGGAGCAACACAATGG
sgRNA2 sequence	AAAGGCGTCTATATCGCCTTAGG

 

left_200bp	right_200bp
GACCCATTGAAAAATACATTATTTATAAAGCAAGTATAGATTTGTATTCTGTATAAGGTATATTATAAAACTAAGATACCCACAGTAAGTGTATCCTGTTGCCAACCTTATTGCGCAGCTGCGAACAGTGTTATTCCTTGGCTAACTAAATCTGAGCTGGAATATCCTGCTGCAATGAGCTCATTACCCAAGGAGCAACA	GCGATATAGACGCCTTTATATGTAAGATTTGTACAATGTTAAGACAAGCTAAGCAAAAATTATGAAGATAAATCCCTAAGATTAGTAGCTCAGTGAGTTATTTTAAACTGTCAACGTTTTATGGCGAATATCTCAATCTTCATCAGTCGTTCATTTTGTGACCCACACGTTTGGCTAAAAGCAGGATGCTGAGCCGGCGC

 

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