CR70053

CRIMIC CR70053

Gene Pngl

CG7865

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:6017739..6020695	42A7-42A8	FBti0220387	94958

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTATAAAAAGGAGATCCAAGCTCTATTTTTCGTG	CAAAATCACTGCGCGGTTCTC

Images

Construct Design Sequences

sgRNA sequence	CGACGAAACCAATCTGATAGTGG
sgRNA2 sequence	GGCAACACACACAACTGTTCAGG

left_200bp	right_200bp
GTTGTGCATCCCCACAGGAAAAGAACATGTACGCTAGTGGAATCCGGCTGGCTTCATTCAACAAAAAAAAACCTAAAAAACACCTCTTTTGTTATCGCTTGAAGTTCGCTAAACTAATCGCGTTTTCCAACACCATAACCCTTTGTGTCTGCATGATTGTCATCCGCCGGCCACCCCTACTAACGACGAAACCAATCTGA	TTCAGGCAGAGTCTTAATTCAAGAGACTATCCCTTCGATCTGCAAGTGCAACTACATTGAAGTCCTCAAAAAATATTTTGGAGAAATTAAAATGTAATTGTAAATAATATAAACGATTCCCAGTCTGGATGAAAATATAATAATCAAAAATGTATATTAAAAATTAAATAAAGGCACATAGTGTCGCTATATGTAATTAT