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CRIMIC CR70079

Gene Hip1

CG10971

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3L:12768432..12773573	69E2	FBti0220390

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAACGAATCGATAGGGTAAAGCCC	ATGGAGCGACAAGGAGGCTAGATT

Images

Construct Design Sequences

sgRNA sequence	CAAGAATTGTCAGTAAACAATGG
sgRNA2 sequence	TTATATCACACCATAGTATGTGG

 

left_200bp	right_200bp
CACTCATTGTTTCATACACGCACACGATCGGAATCCGAATGAATACGAACGCGACCAATACGAAAACAATGGCCAACATTGTTGATAAGATAAGATAACTCCCGTTTCCAATGTCTTTGCTGTCTTTGCTCTAATGCACATATATCGCGCTGCGAATGATTCGAATTTGTTCAGCAAGTACGCCAAGAATTGTCAGTAAA	ATGTGGGTTCATTATTTCTTAAATGTTTATTTTACTTACTACTTGAAATTAAAATAGCACTTATTGCCAAAGAAAATAAAATGTAACATTTGTTGTCAAAAGGTTTTTGTTTATTATCACGAACCGACCTATTGCATTTTATAAAACGGCATGTTCACGTTCATCAAAGACACTTTTAGCGTAGTTTATCTAAAAGAAAA

 

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