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CRIMIC CR70082

Gene Elo68beta

CG11801

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3L:111000675..11101698	68A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GTCACAGATGTTAGTCCGTGGATG	TTGCCTTCCAGTTGAGGACAACGA

Images

Construct Design Sequences

sgRNA sequence	ATCGACGACGTCATGGCATATGG
sgRNA2 sequence	ATTCGATTGCTATATTTACTTGG

 

left_200bp	right_200bp
TATCAATTAAAATACAGTCAGTAAAATGTTAAGTGAAAATGTTATAGGTAAAACCTAGACCTTAACCAAAGTTTATGCTCCAGCGACTTGTAATTAGATTTTGACGAAAAATATCAACGCATCAACGGAGCGATCAAAATGCGTATATAAGTGACTGACCCTTGGCAGTTGGATCTGATTCAAACTTGGAGCCACCATAT	AAATATAGCAATCGAATTTGTTAGTAGTATTATAGAAGGGGTTTCAATTGGTTGACTAATTTAATTAGTTATTTGGGGACACAGAATAAAAAGAACGTACAATTTCTATGAAAATACCATTTGTTTATAGCTTCAATGTGACACTTTCTGTGGTTATAGCATTGTATATAAAAGTATTATCGATTGGCTTGTATTGCTTC

 

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