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CRIMIC CR70089

Gene CDase

CG1471

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:30459521..30465736	99F5-99F6	FBti0216215	92701

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AGGAGGACATCAAGTGCCATGA	GCGTTCGAAGACGTCCATGTAA

Images

Construct Design Sequences

sgRNA sequence	AAGGTAAGGTAGTTATAGCCAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGTTGAGTGTTGTGTGCATCAGAAAATAAATTTAAATGTGTTTAATAGTGCAAAGGAACAAAATACCAATTTTGGAATAGAATACATAAATTTATGTAGTGGATTTTGTTTTTATAATGCACATTTAAGTGGGGTAATTAAACTGAAACGTTACGCAAATTAGTTGTCAGCTCATAAAAATCAAGGTAAGGTAGTTATA	GCCAGGCTATAGGTCATCACTTAATGCGCAACTGTTATTTAAAAATCTAAGTGCACTTTAAGCATATCTAACATGTACATTATCCATATTTAAGGCCACCTTTCCCTATGAGTGGCAGCCAAAGATTGTCTCGGATCAGCTACTAAAGATCGGCGATGTGATCATAGCCGCCGTGCCCTGCGAGTTCACCACAATGGCAG

 

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