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CRIMIC CR70093

Gene Setx

CG7504

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:8131429..8137498	66B7-66B8	FBti0220395

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGTATTCCCTAACGATCGTTCC	GCGTCAAGGCAAGGCAAACAGAAT

Images

Construct Design Sequences

sgRNA sequence	TACGAGTGTAACGTAACAGTTGG
sgRNA2 sequence	CTAAGATATCACAAGTGCCATGG

 

left_200bp	right_200bp
TTGAAATACTTATGTTTCTTATTACGGGAATTTTGTATTTGCATAGAGAAATCCAACAATCAATGCAGTAATTCACTACCACTTGGCAGATTTGTTGTTATAGTAAGTGCATGAAGCGTCGCGCACATTCAATGTTCAATTTTCACTCAAAGTTAGCAGTTTTCACAATTTCCTTCAGTTGAGTACGAGTGTAACGTAAC	CCATGGAAGAGAAGCGCTTCCCACATTGTTTAAAAAAAGGCTTTGTTATTTTTATATATATTTAACATAGAGACTGTAAGATTTAATATTGAAATCTTTGTACATTTAGTTCTTTAAAACGCCGACGAATTGCATTTTCGTTCTTAAATGCCGTACAGTATAACAGGATTTATAAGTAACCGATTATATATCATCTATGT

 

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