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CRIMIC CR70094

Gene lace

CG4162

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:15499127..15505018	35D2	FBti0216216	92702

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTACTTCCGGATGATAGAAAC	GTTGTCGCCATGGTACGGAACTAT

Images

Construct Design Sequences

sgRNA sequence	TTGTCATGCCACGCCATGCCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAGTCCATTTTACCACATGCACTCTCTAATCTAGCCATTGTCTCTTCTTCGGCACTCCTGCACTCATTCGAAATTTTCCTAGTGACTGGAGTACGACCCCAAAGCAAACACACACATAAGTACGTAGTTTGGGTGCACAATGTGTGTGGGGAGAGCGGCACAATGCTGGTCTGGTCATAATATTGTCATGCCACGCCAT	GCCCGGCTCGCCTTATCAGGCCCATTCCACCAGTCTTTTACGTAAAGCCTCGCAGAATGCGATTCGCTGGTGAATCACAATGCTCCGCAATCTCCGAAGGCAGCGGTTTTGGGTGGGCGTTGATTTGTCGGTGATAAGAGTTTTGTTTTAAAATTCATGGACTCTCCCTGCGTCTGGGCTGCCAAAGAATAACTGTTGGC

 

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