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CRIMIC CR70095

Gene Pex23

CG32226

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:20297129..20302141	77B2	FBti0216217	92703

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAACATGAAGGCACATCAGACA	TTGTCATTGCGAATCTCGTCCAGC

Images

Construct Design Sequences

sgRNA sequence	ATTGTTCAATTTATCCAAGCAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACAATACTCTTTACAATGGGTAAGTGGGCTTAAAAACTTACCTGTCGGTCGAAGATTCTATTAAAATTTATGTGGTAGGATTGTTTTGTGAAGTATGTAGGTATTATATTGTTTAAACTGCTATGATAGAAAAATATTTTTGAGTGATGGATAAATCTAGCGCTGGAGTTTTGTTGAATTCAAATTGTTCAATTTATCCA	AGCAGGCAATTTACCAGAAGCTTCGTATTCCATTTAATTCTAAAAGCTTAATTTTTTAATAATTACCTCCTGTATATGTTAATTTCAGAATGCCATGTGGTACTGAGCTAGAGATATCTGGCTGTGTGTACGATGACGCTGATCAGATTCGTTTCGACTTGCAGTCGCATTCTGCAGTAAAAGTTCAGCCTCATCGCGTG

 

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