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CRIMIC CR70097

Gene fwe

CG6151

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:15810081..15816410	72A1	FBti0216218	92704

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	AACAAGAGAGGGTGAGTGAGAGGT	GCATAATCAAAACCCCAACTGCG

Images

Construct Design Sequences

sgRNA sequence	AGGAAATTGCAAGTATGTATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATTTCCAAATGAATTAAGAATGAAACGTATAATTTTAAGGTAAAACAAAGTCCAAAAAAGGTTTTTTTTTGTTTTGTTATTGAAATATAAAATAATACATTCACCCACTTAACTAAGTGGGCTTCCTGCACTTAATATCTCTTATCTAACCATTTCGTTTGATAACTTTTTATTTCGTGTGTAGGAAATTGCAAGTATG	TATAGGAGCTTTTCTGTGTCAAGTTTGGCGCTCTGCTTTTTCTTTCCGCATCGAATGTAATTCTCTGTGCCCATTCAATCGAAATTCGCACTGCCTTTTGTTGTATCAGCAGCTTCCCTCTTTCCATCTCTATTTTTAACTTTGTTTTACCCGTTTGCGTATTTGCTTTTTTATCTGGACTCAGTTAACCTTAACCCTTA

 

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