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CRIMIC CR70098

Gene salr

CG4881

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:11358873..11373636	32E4-32F1	FBti0216219	92705

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCTGTGCTTCCTATTGTGGCCAA	ATGTAGTGCTCTGTTCTGGTGTCC

Images

Construct Design Sequences

sgRNA sequence	CAGGCAATGGAGGTGGGGCTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTCCTTTCCCCAATACCCCCCAATTTTTGCGACAGACTGCATTTGTACATCTTTGGCTGCGCAGCGGCTACGTTCCGTTTTGCTCGCTTAGCACATTGTTGATGATCTAGAGCAGACGAGCATCGGATCGAGTTGGTCCGGGTATGGGGTTATCCCATGCAGATGGTTGCAGGGGGATTGTGACAGGCAATGGAGGTGGG	GCTGGGAATCGGCATCATCTTTTGGGCTTGAAATTTCTAAATGATTCAAAAATCTTAAGTAATTACGAGAATGCATTATGATGATGCGAAATTTACGACTCCGTCTGGTATGTTTTCACTCTGGATCGTGGCGAAATGATGAAAACGAAGGGCTATTCGGGGGCTATAGAGGATCGGGTTGGTTTGAGAGATATAGAGGA

 

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