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CRIMIC CR70108

Gene Lsd-2

CG9057

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:15069127..15076220	13A8-13A9	FBti0216222	92708

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AAGCAAAGCAGACCCAATGGCAAC	CCCACACATGTGACACAAGTACGA

Images

Construct Design Sequences

sgRNA sequence	AGCGGGCAGCACTTATGCTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAAACTTGCACTTTAATTATAGCTAAAATAATGTTACTACTTTTCTTCTCAATATTGATCCCAGCCTAAGTTTGCCGATCGTAAAAACACAGATCCCTGAAAAAACAAATACTTAATGCATTTACTAGACAATACCAATTACCGCTGTCGAATAAAGCTAGATTTGGCTGGAAACTAACTAAAGCGGGCAGCACTTATG	CTAAGGGGCTCTTGGGGTCTCACCGTCAGCAGCAACAACAACAAAAATTCTAGTTGGACTTTCGACTTGTTTGTGATATTTCGCAGTTGTTGTTGCTGCAGTTGCTGGGACTGGAATTGGAATGGATGATGTGTTTGCCGAGTGACGAACTATCAAAGTCTAAACTGGCGGGCCCCAGACAGATAGAATGTAGGGGTCCA

 

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