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CRIMIC CR70114

Gene RhoGAP68F

CG6811

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:12138868..12143380	68F5-68F6	FBti0216224

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGGTACACCATATGGAAACCCA	GGCAGACGATTACTGAACAGAACAG

Images

Construct Design Sequences

sgRNA sequence	GGTTGGGGGTTAGTCAAGGCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAATATGAGGCCAGGTTTTCTACAATACCTCACCTCCAATCTGTGTTTCAAGTTTTGGGAGTCATTTCTTTGCGCTGCTTTATTTCGTTGTTTTCATTGCCGTATCGTAGTTTCTTTGAGGGGGGAAGTGCCCTCGTTTGAGAACTAACAGTTAAAGCCAGTAATTACGACTAATTTTGTGCCCCGCCCCGCCCCGCC	TTGACTAACCCCCAACCGCCGCCCCCTAACCAAGTTCTTTGTTTTTGGTATCAAGTTGCGCTACTAACCCCATTTCAAACTGCATTATCCAGCCTATGTGGGCAGCTTACCGTCTCCTCCTGTACATACATAACTTGCATTTTAAAACTATCCAATGTGGTTTGTTTGCTTGGTTTGTTGTGGATCCTATTTGTTAACTA

 

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