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CRIMIC CR70115

Gene ifc

CG9078

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:5986348..5988854	26B2	FBti0216225	92710

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTACGTGCCAATCGGTTCGAATT	AGACTTAGTTGGGACTGAGAAGCG

Images

Construct Design Sequences

sgRNA sequence	TTAATTGCGCCGATTAGATAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAATAGGGCGTAGTCACACAGTAAACAGCGAAAACATCATCCCATTCCGCATTTATCCATTTAAAAGCCGGCTTGTGTTGTTCAACACTGATTGCTCTCCATTGGGTTTCATGTGAATTTTCACCCCGCAAACTTGTGAATCAGTTTTCATGTTGTTCCCTTTTGCCGGGCATCGAGTGCGTCTTAATTGCGCCGATTAG	ATAAGGTTGTAGTTATATGTGTATCCGAATAGGCGCTCAATTGGTCACGTAGCTCAGTGATTTGCCACTCGACTTCTCGGCTGCAAGTCGCGTTCAGTTGAGTCACTGCTTCGCCTTCCGCAAAAAGTTCCCGAAATATTATTCAAACTAATTTTATGAGTGTTGTTTATGTTTACAGTTACAGTCATTCACTATGTTTC

 

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