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CRIMIC CR70118

Gene KrT95D

CG5405

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:23997538..24031827	95D5-95D8	FBti0216226	92711

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAGTCATCGAAACTGTGCTTAGCC	GAGTGAGTCAAAGCCAAACGCTGT

Images

Construct Design Sequences

sgRNA sequence	GGGGAAAAGCATGCCCCAAACGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTTCCTTGCTGCTAACCCAGATCCGCAGGCACAGATACAAGATACAAAAAGCCGACCCAAAACAAAGACCCACTACCACCAACCCGGCGGCCAAATCAAATGAAATATGTATCAAATATGTATCCCCATAGTATAAAAGAAAAAGAGTGCATATACATACATTTGCACAGATACTTAAGTTCGGGGAAAAGCATGCCCC	AAACGGGGTGGCCAAGAATTTCTGTGGCAAATAGTTGACAATTGTTGGGGCGGCGGCCTTGGCGTGTCGCTCACTCGACTCAATTTGCTTAGATTCGCCCGGTGGCATACTTTCGAACCTATTACTATATCTCAGGGCTATATATCGGGGTCGTGGATTTCCGCATAAGAATATATCCAAGTTGGACTAGATATAAAGGG

 

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