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CRIMIC CR70120

Gene Usp7

CG1490

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	X:12022837..12029032	11A1-11A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
	OK	CCCAAATGTACATAGGCGGATGTAC	TCAAGCAATGAGTCAGCGTCAACG

Images

Construct Design Sequences

sgRNA sequence	AATTGGCGGCACCTCCGCGGAGG
sgRNA2 sequence	GGTGACACGAACCGGAGAAGAGG

 

left_200bp	right_200bp
GCGCCGATAGCGTGCGTATGTTTTACCATCTTGTGCGTACATCATTGTAATTGAACATTTGCTTACGACAGGACTTTCTATACAGTGGGCGAATTCGAAGCTAGAAAAGCAAAGAAGAAGCCGCATAGCGGCAGCAGTCGTCCAGTTAAAAGCAGCATCCAGTGAGTAGCGACAATCCTACAGAGAAATCCGATCCTCCG	AAGAGGAGAAGAATTCACGCTCTAAACTGGATGATGGCGGAGCGGAGCAAGTTTGCGTGCGGAGCGGGATTAAAAGGATGGATTGCGGGCAAGGGATTATGGCCGCACGTGTTTATTGTTCTTCAAAATTCAAAAACAAAGAGCATATTTCTTGGAAACGATTCATACACACCACGGCACACACACACACACACACACAT

 

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