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CRIMIC CR70122

Gene Vps13

CG2093

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:7566809..7580675	43D7-43E1	FBti0216228	92713

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACTACATCGAGAACTTGGAC	CGCAAGGCATTATGTCACCAAGTTC

Images

Construct Design Sequences

sgRNA sequence	AGGAGCATTCAGACTTCGAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTTACTGCGGTACCCATTGTTTATTGGTCTATTTGCACGCAAGTGCCCAGAAAAAACAATGATGGCGGTGACCTTATCCTTGGCAAAATACTATTAATGACCTAATTTCGTCGCTTCTAAGTGAGCCGTATTCGCAAGTTCATGCGAACCCAATGAATTCTTCTGAGAAAGAGAAAAGACGGAGGAGCATTCAGACTTC	GAGGGGTGGGACAACTGTTGCATCTTGCTGTGCGATAGAGCAATTCTTGTGGTTTCTCTTGGCTCTCCATGAGCTCATTTAGTCTCTGCCTTTATATCTTTTATTTACAAATCTACTTGTATAGGGTTTCTGTGTGAAACATAAACAATTCTTCGGTCTTTTATCTGAATGTACTCACTTCACTTCATGTTAAGTGGGTT

 

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