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CRIMIC CR70124

Gene vps13b

CG15523

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3R:29935978..29948207	99C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GCGTAACTTTAGACCCATTCTTAGC	AACCAGGAAGGATATCGAGCACAC

Images

Construct Design Sequences

sgRNA sequence	AGTCAACAGCGCTGGGATCGAGG
sgRNA2 sequence	CATAAAATACACAGGATTCTAGG

 

left_200bp	right_200bp
GCACCTTCCGATAACGATAACGATTGGCAGATTGCCGGGGCTGAAACGTAAACAATCCAAAATTCTATGGCTATGTTTGTTTTGTTAAACATATTCGGCCCAGAATTGTAGCAAAATCCACAGCATGTAAATTCAAGTTAACCGCCGGCAACGCAGTAAATGAAGAGTGTCATATAAGTGAGCAGTCAACAGCGCTGGGA	ATCCTGTGTATTTTATGCCAAAAAAGACATTTTTTGTTACAATTTTTGCAATAAAAATTAAAAAGAAAACGTGTCTTTTTTCCTAATGACTGCTGAATTACAAGGTAATTTTTCTCTGGAACTAGTGTATATTATTTTCCTCTGCTTTTACTCAGTTATTCTATGTGCTGTCCAATTCCGCAAAATCCCATCAATTCCGT

 

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