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CRIMIC CR70127

Gene CG33090

CG33090

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:14334158..14345795	35A4		92714

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TCACGTGGACAACAGCAACATCCA	TGCAGACAGCAACTGCTTACAC

Images

Construct Design Sequences

sgRNA sequence	GTCTTGTCCCTGGTGCTGGATGG
sgRNA2 sequence	ATCTTTGGTTGTGTAGACTTCGG

 

left_200bp	right_200bp
CAACGATTCTGGGTTATTTTGGGTTATCTGGCGCGACAACAATGATTTTGTAACGATATGGTATATATATGGTATATATGGTATGGTATGATGGACGTTTTACCTTGGTTTTCTTTCTTATGCACTTTGGTTTCGATTTGGTTTTGCTTTATTGCTTTGCCACTACCGCGCAATCACACACACCCACACCATGTCCATCC	TCTACACAACCAAAGATATTTCTAAGCCAGCACAAAGATTTTCTTAGCGTTTAGTTCGATTCTTCTTTTGAGGCTGAATGTGTAACGAGCTTATCGAGATGGTGCGAGTCGAGGAAGATGTTGTCTCTGAAGTGGTTTTCAAATTTATAGCTTTATCTGTACTTTTAAGCTGTGGAATCTGTTGGCGCTTACTCCATGTC

 

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