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CRIMIC CR70130

Gene mAChR-C

CG12796

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	X:6831397..6832684	6D4	FBti0220405

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGTTTACCACGTATCCCAGTTGC	GCGACGTTTCACAGACTTGTTC

Images

Construct Design Sequences

sgRNA sequence	TCGTTTACCATCGTGGCTCATGG
sgRNA2 sequence	GAAGGCCACTCAGTGATCAACGG

 

left_200bp	right_200bp
AACTGGCGCGATGACGATGTCGTAAAGCCACCAGCGAAACGTAAACAAACGACGCCCACGGTCCGAGCCGAAACCCAATTGAATCTGAATCCGATCCGAGCCGAGCCGAAGTCCTGGCCAAAAGTCAGCACAGCTCCAGCTCCCACGGTTGCCAGATAGCCAACCACTGGCGTCGCGTGCGCTTCGTTTACCATCGTGGC	CAACGGAATTGGGAAACCCAAAGCTTTAACTTGGTAGAATTAAATGGAGATATAGCAATAAAAGTGGACATTATCTTTAGGAGCACCGTTTCTTCAACACCCCCAGCAGCGATTTGTTGAGATAATTGATTGGTATAGGATGAGCAGTAGTCTTACCTCGATTCCTGTGATGCCCATGGCCTGCACTAGATTCTGGGTAA

 

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