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CRIMIC CR70135

Gene srl

CG9809

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:4422483..4429335	82B1	FBti0216229	92715

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGTTCATCTTACGCTGACTTGG	GCGTTCCTTGCTCTCTCTCAAA

Images

Construct Design Sequences

sgRNA sequence	CTGTCAGTTGCAGTCTGAAAGGG
sgRNA2 sequence	TTAAAAATACGAAATTAAGCCGG

 

left_200bp	right_200bp
CCCTATTTAATCCGAGTTTATTTGTTTACAGTTACTTAACTTTTGACGCAAAATGGATTCACGAATGCTAAATGTGTTCCAAGGAGATACCTTTGAGGCAAATTATACCAAATACGAGTCTGAGCCGATATTCTGGTCATCTAATGATGAGTGAGTGATTGTCATCAGAAAAATGTACTAAAAACTAATGCAAACCCTTT	TAATTTCGTATTTTTAATTTTTAATTTTTTTGAGAATTTGAAGTATGTTACAAGCATTCTACCACTATCCTTGCACAAGTGTAGACCAATTTCCGAAGTCTCTATCTATAAACCTGAGAAGCCTGATTACAATTCTTAATTTATCTTAAAATAGTTGACAGAGCTATACAATGCAAGCAATAAAAACATTGCAATGTACA

 

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