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CRIMIC CR70136

Gene Root

CG6129

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:24113286..24128193	95E1	FBti0216230	92716

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAGCCGATATCAATAGTGCGGTA	ATACGTTGGATCTCCAGCTCCACT

Images

Construct Design Sequences

sgRNA sequence	GCGTGAGTGGAAATTGCAAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTTCCGAGTGTGGATGTCAAGGCGGATTGGGGGCGGATCGTTGATAACTCGATTAGTCTGCGTCTGCCTCTGATTACGCCGTAGATTGTATATAATAATGATGTTTCCAACTCAAATCCGGGGTGATGCCATGTCTGCGATGAGTTACAAATTATGGCTGCGAAGATTGCATTTGCAGCCGGCGTGAGTGGAAATTGC	AAGGGGATAGGTTCATCAGCACCAAGATCTGGTATCTCTAATGGGGTTGACTGGGATCGGTTATAAAGTGGGCATAGTATAGAGCTAGACTATATCAAAATGGCGTTAAATGCAATAATTATATATTTCTAAGTAAGCAGATGTTACATAGTTATGTAATTATTTCTAAAAGCTGTCAGTGGGCTTAAAAAATAAAAACG

 

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