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CRIMIC CR70139

Gene wfs1

CG4917

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3R:22687542..22690380	94C1	FBti0220407	94979

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCTTCGTGCACCTGGTGAAGATT	GATCTGATCATGCTGGGAGTGGAT

Images

Construct Design Sequences

sgRNA sequence	TTCTAGTCTTGACTTAGCGATGG
sgRNA2 sequence	CGCCTCCAAGATTTGATGAGAGG

 

left_200bp	right_200bp
TATTTATCGATTAGAAGTCACGACATTATACACAAAGAACACAAATTTAAATATAGGAAATTATTTATACTACATAAATGTATTTTATATACTATATACGATGAATAATTAAACTTTGGTAAACAAATTTTTAAATTAATAATTATATTAACTAATATTTGTGAGTCCATCTCTAAGACAAGTTCTACACGATGCCATCG	GAGAGGCATCAAGTATTTGTTGAACGCGTTGTTGAATCCTTTAATTACATTTAAGTAATTTTCATTAAATTAAAAAACACAGGAGTATGAGTTTCTTGTAATCTGCTCACCCCATTTTTTTTACTTTAATATAATAGATACATATGTATTTATTTAAACTGATAAATATTTACCAAATTATATTGGTTGATTATGATAGT

 

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