CR70140

CRIMIC CR70140

Gene cnn

CG4832

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:29123370..13450475	50A8-50A9	FBti0216232	92718

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CCACTGCTTTTCAAGCAGCCTATG	TCCTCGTGATGAGAAATGGCTCGA

Images

Construct Design Sequences

sgRNA sequence	GCATCTGCAAAGGAAACAAAGGG
sgRNA2 sequence	CATTGGGTGAATGGCCTCTCGGG

left_200bp	right_200bp
ATTAGCACATTTATTCGGCCGCACTGTTGGCGTTTTATATTTTATATGTGGTTGCTCCCTAACGGCTAGGAGAAAGCTATAAAATCATGAATAGTAATCGAACGTCGTCTTCGCACAATTCGAGAAATCTAAAGATTCTTAGTCAGTAAAGATAACCTTATGGATTATCCTAGACACTCTAAATCAATATCAATCCCTTT	AGGCCATTCACCCAATGTTCAATTGCCATTTCTTGCCATTCTTATATTTTGTATGCATTGTATTTTGTTGTCTCGTTGCTAACTTGATATCAATTATTTATGTGTTTTCTTCTATTTTTTTTTTTTGTTTAAAAAGACCGTAAACAACGAAGCTATTAGTACGAAATACCCATGCATTTTCTAAAGACTGTTGAGGGAAT