CR70142

CRIMIC CR70142

Gene ctl2

CG11880

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3R:29123370..29128247	98F10-98F12	FBti0220408	97732

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	TTCACCAGCCCATCCAAAGCCATT	GGCTTCAGCGATCTGTTACATCCT

Images

Construct Design Sequences

sgRNA sequence	CTTTCAGTAGAGAGCGATGACGG
sgRNA2 sequence	TTGCTCAACCGGCTGCAATTGGG

left_200bp	right_200bp
GTTGGCTTCACCGCCAACACGACCACCCACTACAGATTCGGTTTTCCATTTTTAAAACAATGACGCATGCACGGAAATAAATTACATGATATACGCCTGACAATCTCATTACCTAGAGACGACGACCCATCGATAGTGGCTCTATGTATATACATACATAAAACACTCGGTTCTTCTTGGACTCTTTCAGTAGAGAGCGA	ATTGGGTTTGCATTAAATGCACGCTCCTTTGCCAATACTCTTAATCTTTAATCTAGTGTATATAATACGCCAGTATCCGTATCCATATGTAAAGTTTTTTGTGCGGCTAGACATGTGATAGGCGCGGACCATAAAGACCAAAAGTAATACCATACAATAACATGTATACAGAGACACAAAAGTACATATCTCACATTCAT