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CRIMIC CR70143

Gene CG8778

CG8778

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:12651301..12652487	49B11	FBti0220409

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGTGGAATGCTCCAATCGTTCCT	CAGGTGACAGAACCCAGTTGTTTG

Images

Construct Design Sequences

sgRNA sequence	TAAGCTGATTATAAAACGACCGG
sgRNA2 sequence	TCAGCTTAACGTTAGGGGAGTGG

 

left_200bp	right_200bp
AATTCTTATAAAGCGGCTTATCTTTATATCCATATACATTGATATTACTATTTTATATATTTGGTCAGAAGAATGCCGTAAAATGTTAAAGCAGTGTGAACAACCGCGGTTATTAACCGCCTTGGTGTCTTTAATACTGTTATCGATAGTGGCTTTAAGCTTTTGTCAGCACCAGCGGCAACAGCTGTTTTCTGCCGGTC	CCCTAACGTTAAGCTGAAAATTGCATTTATTATTTAACCTGTTACTGTTGTAGAAACACTTAAGCGGCAAAGCCCAGTTCAATTGTTTTTATTAAAAGCATTCGATCCTATCGAGCTGCCAAATAACTTGAATGACGCTAATTGTAGCTAAACAATTTTTAATACCTGTGATGACGCATCTCAATTTAGACATGGGTGGG

 

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