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CRIMIC CR70144

Gene Adk2

CG11255

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:13019715..13022688	69F5	FBti0216233	97729

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGCCCAATGGATTCTTCGACAGC	AAGATGATGTCCACGTAAGGCAGG

Images

Construct Design Sequences

sgRNA sequence	TAATAAATTAATGGCACAAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGGACGTGCCCACGGGAACCTGTGCCGTTCTGATCACCGGCACCCACCGATCGCTCTGCGCTAATCTGGCGGCCGCCAATAACTTCACCATCGATCACCTGGAGGAGCCCAGCAACAAGGCTCTGGTGGATAACGCCCAGTACTACTACATTTCGGTGAGTTCTGTACAATCGATGCCGGAAGTAATAAATTAATGGCAC	AAAGGGAAGTAAGTAGTTAAGCGATTTCTAAAGAGTTAGAGAGATTAATCAGAGATATTAATCAGAGTTTAGTTCCTTAATTTCACATTAATTTTGTTCTTACCTACCTAAAAAATCTTTACTTAATTTTACTTCCCTTGCTTTGTTCTTTAAAACATAAAGATATCGTATGTATAATACTTCTTATTATTTCATTTCCC

 

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