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CRIMIC CR70145

Gene CG8199

CG8199

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:9555785..9557776	85D24	FBti0216234	92719

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACTTTGTCACCATCTCCAGTCCG	TTTCGACAGTCTCGTCAAACCAGC

Images

Construct Design Sequences

sgRNA sequence	ATAAGAAATACATCCAAAATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCCCAGGCCGTTGGTGCCGCCTACGCCATGAAATTGAGGCCAAACAACGATGCCTGTGTGGTCTGTTACTTTGGTGAGGGAGCTGCTTCCGAGGGAGATGCACATGCTGCCTTTAACTTTGCTGCCACTTTGGGTTGTCCGGCTATCCTCTTCTGGTGAGTAACTTTAAGATACATATTATTTTGATAAGATAACCTATT	TTGGATGTATTTCTTATCTTATCAAAAATACCATTCAAATAAGTTTCTTCATGACGTACCTTTCTCCACAGTCGAAACAACGGCTTTGCCATATCCACACCATCGCATGAACAGTACAAGGGCGATGGAATCGCCGGTCGCGGACCTATGGGCTATGGAATTACTACCATTCGAGTGGATGGCACAGACGTCTTCGCCGT

 

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