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CRIMIC CR70149

Gene CG5044

CG5044

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:15357229..15359263	88F1	FBti0216958	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGCAGCAAGATCCCTGATCT	AATCACTTCGCTCCAGATGACGCA

Images

Construct Design Sequences

sgRNA sequence	AGCGCGCGTCAAGCGATGATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCCCCGCCAGAAAAGCCCTTCTCGCTGCAGCCTGTCCTGGAGCAGATCAACAAGAACTTTTCAGCGGACTCGGTGGAGGGGATTCTAGAGAATCTGCAGAACGATGGCAGTGAATGGGCCAAGAAGACGCTTGAGGTGAGTGGGGCACAGTTTACTAAGTGATAAGAACACAATCGGATGTTAATTGGCTGGGCCAATC	ATCGCTTGACGCGCGCTTTTAGCGCCTAAATATTTGATCCGTTTCCAGCCAATTAGCAACGCTTTAGTTGCTGCACGACTTTTCTTAACTGAATAAGTTACACTAGCTCACAAACTCACACCCCACACCTTGTTGCATTTTTCTATTAGAGTTGAATTTTATTATTGAAATCAGACACATTGCTTTTTTCTTTGTTTTAG

 

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