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CRIMIC CR70150

Gene subdued

CG12298

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:19633418..19642722	92A11	FBti0226855	97156

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATGCCCATGAAGGAGGTAAGTA	ATCCTGTCGGATGTCAAAGCTACC

Images

Construct Design Sequences

sgRNA sequence	AAATCTATCAAGGTTAGGCTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGAAAGAATTGGAAAACATGTTTAAGACTAGAAAACACGCTTTTGGTCGATCGATTGACCACGGACATTCCTGGAATCTAAAGGCATTGAAAGTTCAAGTGTAAATGGTATTTAGAAACTAATTTTAATAATTTTGTGTTAACATTCAAGCTGGCTTGTTCTAAGTTACATTTTCTTTATACAAAATCTATCAAGGTTAG	GCTGGGTATTTTAGTTCGATTTCAAACAACTCACAGCGGAAGCTAAACGAAATATGATAATCTAAGATTATTTTTAGGGGATTAATAATAAAATATTTAACTGGTTTAACTTCAACGCTTTGGCCAACCTTATTGGTCAAGTAATGGGCCGCATTTCGTAGGCGATTCGATCTTTCAGATATGCGTAAATTGCATAACGA

 

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