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CRIMIC CR70151

Gene Tusp

CG5586

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:27607879..27634932	98A12		92720

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AGCTCCTAATGGAGCCAGAATCTG	GGCTCATTCCACTTGACCAGGATA

Images

Construct Design Sequences

sgRNA sequence	GGCTAGAGACCCGTCGTCCCTGG
sgRNA2 sequence	TCCGTCCATCCCACCGTGTCTGG

 

left_200bp	right_200bp
CATGGTGAGCTGCTGAGGCATTCCTAGGAATCCACAACAGTTGAGTAAAAAAGCCCAGCCTCGGGCTGTGAAATAGGCAGCTGGCTTGGGTTGATCGGCTCAAAGAGTGAGGAATAAAGTGATGCAGTCGGAAAGTGGAGCATATTTACCAAGCCAACCGGCCGCCTGTCGATGGTGGAAGATGTGTGAATGGGCCAGGG	ACGGTGGGATGGACGGATGGTTAGCGAAAATTGCATCTTCATTTGTATATATTGTATATGTATATTGTGGTCTTTAGGTTTCCGAACACCGTACTTTAGTGATTATGTATGTGAACACGTACCACCACTTGATTGAAGGTTTTGCCATGTGCTGCACATTGAAACACGAGTGCGCACATGGGTGTGCAAACTCACAGTCC

 

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