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CRIMIC CR70155

Gene Cln3

CG5582

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:17809231..17814119	75A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAAGACTGCTTACGCACATGCTC	CATCTCACTGCCAAACCGATCGTT

Images

Construct Design Sequences

sgRNA sequence	TGGTTGCCGTCATATTGAGCCGG
sgRNA2 sequence	TTGTTTGGTGATACGGATCGAGG

 

left_200bp	right_200bp
GTAAATGCTAAAGCTTTTAAAAAGAGCAACATTAATTTTTTTAGAGACAAATAAGTTGTTGATTTCTTAGGAGAAACTAGATGAGTTTAGAATAGAGATAGTAGATGGTAAAAAAAATTATGAAGTGAATCAAATAAATTTGTTGGATTTGCACTAAAAATGTTAAACAAATGGCTTTTTTAATTTTTGTTTAGCCGGCT	TCGAGGATCAGAATCAGGATCAGATTATGCCTTTACTGTGTGCGTGTTTAGCCAAATAAGCGTGTGTGTCTGTGCATGTCCCTATAGTATTAATCACTGGATCACTGGACATCCGGTAACCAGATAGATCGTTGTATAGTTAGTATGCTGCTGTTAGACTACTCTATAAGTTTAGTTTAATAAAAAAAAAAAATAAACAC

 

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