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CRIMIC CR70165

Gene PolA1

CG6349

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:21666977..21666978	93D-93F2	FBti0228265	97726

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACACACTCCTTGAGGAAGAA	AGCACTCGGAATTGCTTCGATCTG

Images

Construct Design Sequences

sgRNA sequence	ACTGAAATCCGTCGAGATCACGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATGTTCGACCTCGGCAAGCAAACGCTGCAGCAAAAACGTAAGTAAATACAATGTAGTTATAAGCAGTATCAAAAACTAAAACAATAGTTTGTGTATAAGAACAAAATGAAGAATGTGGAGTACGAGAGAGAAATATCGACACTAAATAACAAGACACATGAAAGGAAAAATCAGTCGAAATATTTTATAGTTCCCGTGA	TCTCGACGGATTTCAGTAGTTCTTTATGCTTGTATCCTTGAATTCTTAAAAATTGTTGTAGTTTATATATTAGTTCTGACACCTAATACATATATTTCCCTTAAGATTGGTATCATTAAGTTTTACAGCATTTCATATTGAAATTATATTTTGTCCAAATTATCAAGCCACCCTCACACCGGAGCTGGAGCAGGCCTATC

 

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