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CRIMIC CR70167

Gene rhea

CG6831

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:8545001..8574409	66D6-66D7	FBti0216237	CR70167

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTCTACGTCTATTCTTAGATGC	ATATGTAACCCAGCAGAGCTCGCT

Images

Construct Design Sequences

sgRNA sequence	AGTGTTAAACGTATGTGCAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTCTTATATGGAGAATGAAGTGCAGACCGTGCAGTACGGTGCCTTCGTCGGCCAGGTGAACCATGCCCACCAGCCGCCTACGGTGAGTAAACCACATCTCCATGCTCAGCCCAAAGCCACCACGCATTCTTTAATAACTCCATTTATTTACACTCAGCCATTTATTTATTTCACACGCATTTTGCTTAAGTTGCCACTG	CACATACGTTTAACACTAGCTTACAACTAAATCTGATCCACAATACCATAATCCCATCCATCTAAAGCAAAACTAACATGAATCATTTCTCTAATCCTTTCTCCTGACATGCTGCAATCTCAAAATAAAGCCAGTGGTCATCCAATGTTTTACAAAGTATGCATTTCGCTGGAGAGAGTTTCTTCCAAAACAAAAACTAA

 

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