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CRIMIC CR70168

Gene PK2-R1

CG8784

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:13338330..13345106	87E6	FBti0216961

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAAGTCCGATTACAAAGTTAAGGC	AAGGACACTCGATGATGCGTCAGT

Images

Construct Design Sequences

sgRNA sequence	AAACGTGGGCAGATATAAGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTCACTTTACCAAAAGAAGTTAAAAGTGATGTCTATTAAATTTGGTAGGAAACTGTTTGAATTCCTTCCCATTTACACCCAAATTCAAAGTAAATATTTATGTTGCATTCAATATCCAAAACCTTAAAGAGAAAGTTAAAAAGCGTAAAAAGTGTAGGTGGTCACTGCGTGTACTTTACAACCGTAATATTTTCCATCT	TATATCTGCCCACGTTTATCCGAAACATCGACATCATCTTGTCCTTAATACTTAACATATAATGTAGCCTAATCTTTCCGTCATTTGTAGGCAGCACACCATGTCAAAATTGTCGCGAGCCATTAAATTTATATTTGCCATTTGGCTGGCGGCCTTCCTTTTGGCCCTTCCGCAGGCCATGCAATTTTCGGTGGTCTACC

 

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