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CRIMIC CR70169

Gene rhea

CG6831

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3L:8545001..8574409	66D6-66D7	FBti0220418	97731

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	AGAGCTTGGCTCATCCAGGCATTA	GAGTGTATGAGTAGATGAGTGCC

Images

Construct Design Sequences

sgRNA sequence	GAAGCAGCGGAGACGACGCCAGG
sgRNA2 sequence	GGTATAAAATGGGACAGGTGCGG

 

left_200bp	right_200bp
TATCCCTTAATATAAATTATCGCTGTGTACTTGATTAGTTGCTAAAAATTAACCAGAAGTTCTCTTAAACCTTATTTTTCCAGTGCCCATTGCATTTTTCACTTCCTTGGAAGTTTACCCAAGGAAACGACGACGAAGCGGAGTAGAGAGAAGTAAAAGAAAAGGAGCAGCGGAAGTCGAAGTGAAGCAGCGGAGACGAC	CTGTCCCATTTTATACCCCCTATCTTTCTCTATTACTCTCTCTCTTTCTTGATCTTACGTAGTTTTTAAACTCTTGCTAATAAGCTTTCGTTGTTGTGGCTTTTAGTATCTGCAGGGTGATTCCCTTAACTGATTTTAAACTATCTGAAACATGTAGCTTTCAATAATGCAGTTAAGGAAATTATACTGCTTTAGTTTCT

 

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