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CRIMIC CR70173

Gene LKRSDH

CG7144

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:7791175..7795959	28C4	FBti0216238	92724

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGAATGTCAAGGTCATAGTGCAGC	CTATTCATCCTGCTACGTGGTAATC

Images

Construct Design Sequences

sgRNA sequence	TTTGTACTCGATTTCAAGGCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTATTGAGTCTGCGGGGCTAATCGCCCATGGAAATCATATCGGTCGGTAGTCGTCAGGGCAACAAGTACGCGTATATAGGTAGCATAGTATGGTATATATATGGTATGGAGGCACACACGCCCCCCAAGTGCTGCATCATAAATATAAACTCGGATGATAAGCCTCCATGGAGCTCTAAATTTGGCAATTGGCCCGGCC	TTGAAATCGAGTACAAAACAAACGACGAGGAGAGGAAAAACAATGGGAAGCAAAGCTTATCGTTTGCAGCAATGTTTCCAAGGAATCTATATAATGTATTAAGGTGATTTTTAGAGATGATAACATTTTCAAAGGGTCACGTGAGAAAGAACTAAACTAACTGAAATAATTTTTGAAGAAGGTAAAGCACTTAATTTAAA

 

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