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CRIMIC CR70174

Gene CG7414

CG7414

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:21824740..21827856	78F3-78F4	FBti0216239	92725

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTGTGTGACCAATTCAAGCACGA	GCGAGAATTGGAGGTAGAAGGCTT

Images

Construct Design Sequences

sgRNA sequence	CGATTGGGCCGCCCGGTTAGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAACAACAGCAACAACACCACCGACGTGCTTGTTCCTGTGTGTGTGTGCGTGTGAGATTGGAAGACAATGGCTGCAGCTGCGGATCTGGTTGTTCCTACCCTTGCCAGTGAGTATAGCCCGGATGCAATCCCCGCCAGGTGGTCACTGAGGTGCCGTCGACTCCTTGGAGCATCGAAGGCAGCCGATTGGGCCGCCCGGT	TAGCGGAAAAACTGGAAAAACCGCGCGACCTCGTGTTTCCACCTGCTAATCGGGGTCACCTCTCGATTCTCATCAACAGTTCGCTCTAGCGTGGCCGTGGAATTGTGGTCCTCGGCGGGCTCCAAGCAGCCCTTCGAGCACAAGCCCCACCTGCCGCGCGAGGACACGAAGAGCTCGCGCTCGATCTGCTTCAGCTCCAA

 

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