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CRIMIC CR70176

Gene Loxl2

CG4402

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:21789623..21791580	58A1	FBti0220419	94990

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACGTGCACCGAACAAAGGTCATA	GATGAGTGGGGTTAGTTGCTTGTG

Images

Construct Design Sequences

sgRNA sequence	CAGTTCTCATGCGAACGCCGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGCCCTCGTCGGGCGGCCAATGACGTCAGATGAGCGAGCTTCTGAACCGCATCAGATATCTGAATATGTACCTAACAAGCCACCTACCTACCTACCCATCTAGCCGTCTATCTATGTATCTTTGTATCTGGCCAGTTCATAGTTAAATGATTCTGTTGCCATTCAAATATTTGCGTTGAGCTTCAGTTCTCATGCGAACG	GGGTGGATGGTTAGGATTAAGTGGGCGGTGGACTGGAACCAAATCAAGAAGTGCCAAATACAACGATTAACCAAAGGGGGCTTGTATATTTTCTACACCTATGTATGTCTTTAACCCTAACTCGCATGCGATATAATTAAATGTGCACATCTGAAAACTCTTGAACTGAACTTGAAAGTCGTTGGGGTGGAAACATGTCA

 

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