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CRIMIC CR70177

Gene CG12268

CG12268

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:23943628..23948424	95D1	FBti0216962	93368

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGACATACTGAGGAGCGATG	CTAGGGAATTACGAAAATAGCTTTAGTC

Images

Construct Design Sequences

sgRNA sequence	ATTAAGCTGCTTAAAACGAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGTAGCTGTGCCGACCTGAACGTCATATATCTATTGATACGCACCAAAAAGGGGGTCGATCCCAGTGTGCGCAAGGAGCAGTACTTCAAGTGTGTGGTAAGTTTCCAGATTCGCCTCCAGTTACCGGAACTCAAAGCATTCCATTATTAAATGCTGTAAATTGAATAGTTTATGGCTCGTAAATAAAGCCCAGCCCCCTC	GTTTTAAGCAGCTTAATTAGCAGCTACTTAGATTGCCATTCTCCGTTGTACCACATGACGTATGCGTAATGGCCATATAAAGTGCAACGAAGTGCATCAAAATTCCATGGAATCGTATAATGTGCTATGTTGCCAATTAAAACTAACAATATTGATTTGTGTGATTTGGATTTTCTAATAATTACTAGCTGAAGCAAAAA

 

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